Coding

Part:BBa_K106002:Design

Designed by: Ryan Quan   Group: iGEM08_UCSF   (2008-10-22)

Sir2, Aar1 AB part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 823
    Illegal BamHI site found at 261
    Illegal BamHI site found at 809
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this is an AB part, there is no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.

Source

Yeast genome, with 2 internal AarI sites mutagenized (conservative).

References